A strain of high-efficiency bioflocculant-producting bacteria, which was named TJ-3, was screened from sewage sludge. The strain was gram-negative and rod-shaped in physiological biochemical test and was identified as Pseudomonas aeruginosa by 16S rDNA Sequencing. According to the growth curve of the TJ-3 strain, the growth stabilization period is long so that Microbial Flocculants (MBF) production is stable. The MBF produced by the TJ-3 strain is able to flocculante kaolin suspension with 98.2%. The MBF activity distribution tests show that most of the active components of the bioflocculant exist in deposition after centrifufation. When pH is 8.5, 1% (quality fraction) CaCl2 Solution dosing quantity is 3.5 mL, bioflocculant dosing quantity is 1.5 mL in 100 mL kaolin suspension, the bioflocculant has an optimal flocculation.