Utilizing the enrichment substrate, an aerobic denitrifying bacterial strain with the capability of phosphorus removal was screened from the activated sludge which had been domesticated by actual living sewage. By the morphological observing and identification of the physiological and biochemical indexes, the strain was identified to belong to Pseudomonas. The aerobic denitrifying bacterial strain was applied to deal with the simulated and actual living wastewater. By inspecting the changes of total nitrogen, inorganic phosphorus and CODcr in the wastewater, it was ascertained that the optimal wastewater treatment conditions of the bacterial strain were C/N= 3, inoculated ratio= 10%, pH 6.8, 30°C, treatment time= 2 d. After the activated sludge was strengthened by the aerobic denitrifying bacterial strain, its treatment capability for actual living sewage was improved, evidently.