A strain JY01 (Bacillus sp. JY01) isolated from wastewater of insulating materials plant had strong ability of phenol degradation. The morphological and physiological characteristics of JY01 were almost the same as Bacillus. The sequence alignment algorithm of the 16S rDNA gene of JY01 shared 99.01% similarity with the typical strain Bacillus simplex (AM921637). Under the conditions of incubation amount of 2%, it could preserve high degradation activities of phenol in the range of the pH 6.0~9.0 and the temperature 18°C~36°C. With the phenol concentration of 1100 mg/L and 1300 mg/L, the degradation rates were 99.16% and 74.76%, respectively. The experiment offered a comparatively reliable parameter for the phenolic wastewater treatment.