Actinobacterial community structure in a soil sample from Yutian pit in Xinjiang was investigated by using Culture-independent method and phylogenetic analysis based on 16S rRNA gene sequences. The result showed 41 clones distribute in 26 OTUs, which exist in Acidimicrobidae and 7 suborders of Actinobacteridae respectively, especially the dominant community of Yutian pit was streptosporangineae instead of streptomycete, which accounting for 42.3% of sequenced clones. The similarity between 71.8% of 41 detected sequences and published clone sequences were less than 97%, which represented a new community and 15.3% of the clone sequences which identitied with GenBank clone sequences were under 85%, which may represent some new families or suborders. The results indicated that abundant actinobacterial community and new actinobacterial resources existed in Yutian pit. In addition, actinomycete community which exist in different high salt concentration environment have distinct differences for microecological effection.