Aspergillus oryzae L-09 and Saccharomyces cerevisiae Z-06 were mixed cultured, and the component of the submerged culture medium was optimized. In the optimum medium, the activities of the α-galactosidase and invertase reached 85.22 U/mL and 563.44 U/mL, which were 5.9 and 8.3-fold higher than for the single strain, respectively.