The spermosphere as an important habitat in plant micro-ecosystem is rich in temporal succession of microbial communities form and function. The indigeous and inoculated microbial community diversity, proliferation and activity could be regulated by the temporal succession and exudation of germinating seed exudates. The microbial chemotaxis in spermosphere is essential for their colonization , antagonistic activity to pathogens and bio-control of plant diseases. It shoud be assured that all the determination carried out are within the ephemeral time frame of germinating seed exudation and microbial activity with the unique experimental conditions in studies on the spermosphere micro-ecology. And some new insights would be proposed for the origin of microbes in rhizosphere and the reasonable application of benificial microbial inoculants in further investigation with the culture-independent as well as culture-dependent approaches in this area.