According to teaching request for medical microbiology, blended teaching mode basing network sources was practiced in the course of clinical medical specialty, i.e. 80% of total class hours was used to traditional classroom teaching, while 20% was used for network classroom teaching. The examination and questionnaire survey were conducted after the end of the course, and the result showed the teaching effect of Blended teaching mode was much better traditional classroom teaching. Furthermore, blended teaching mode was helpful for overcoming limitation in traditional classroom teaching and training the self-study ability of students.