Bacterial biofilm is a biopolymer matrix-enclosed bacterial population adherent to each other and surfaces or interfaces. The organisms within biofilms are notorious for their resistance towards antibiotics compared to their free-living planktonic counterparts. Consequently, biofilms are of significant importance to both clinical and veterinary science. However, although antibiotic resistance of bacteria are widely reported in animals, their association with biofilms is rarely discussed. The aim of this review is to look at the mechanism of antibiotic resistance of bacterial biofilms and discuss the relevance between antibiotic resistance of animal bacteria and biofilms, besides this article can be seen as a reference in studying antibiotic resistance in bacteria and guaranteeing the safety of animal products.