The adhesion of 9 pig Lactobacillus strains on IPEC-J2 cell line and the effect of 5 Lactobacillus strains on the competitive, exclusive, displacement of adhesion and invasion of Salmonella typhimurium DT104 to IPEC-J2 cells were investigated. The 9 pig Lactobacillus strains exhibited dose- and strain-dependent adherence to intestinal epithelial cells by 0.1%~10%. The ability to inhibit the competitive adhesion of Salmonella typhimurium DT104 appeared to depend on the dose and Lactobacilli tested. Salmonella typhimurium adhesion was reduced above 80% by 109 CFU/mL K30, K67 and K16 strains. Adhesion percent of S. typhimurium DT104 was reduced 40%~70% by pre-treating the cells with 109 CFU/mL Lactobacillus prior to adhesion to IPEC-J2 monolayers, while invasion percent was reduced 23%~33% by 108 CFU/mL Lactobacillus. However, no inhibition of displacement was detected at low concentration of Lactobacillus, while 12%~84% displacement was detected with high concentration. Therefore, the observed inhibition of S. typhimurium adhesion and invasion to IPEC-J2 monolayers with Lactobacillus could be efficient for preventive or curative probiotic therapy in pigs with S. typhimurium disease.