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一组多功能细菌复合系NSC-7的培养特性及稳定性
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国家“十一五”支撑计划(No. 2006BAD25B04); 国家自然科学基金(No. 30571088); 东北林业大学青年基金(No. 07035)


The Cultural Characteristics and Stability of the Dualfunctional Bacteria Community NSC-7
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    摘要:

    本研究探讨了一组具有分解纤维素和农药林丹双重功能的复合菌系NSC-7的培养特性和稳定性。NSC-7在14 d的培养过程中, 使稻秆分解73.6%; 用GC-MS测定结果发酵液中检测到10种化合物成分, 其中峰值较大的依次为乙酸、甘油、丁酸、丙酸; NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性; 经90℃高温处理30 min后, 仍能够保持分解能力, 105℃处理30 min后转接2次就能恢复分解能力, 显示出很高的保存稳定性和热稳定性。利用DGGE分析多次继代接种过程的培养物结果条带基本没有变化, 表明NSC-7的菌种组成稳定。

    Abstract:

    The cultural characteristics and stability of the bacteria community NSC-7, which was capable of degrading cellulose and lindane with high efficiency was discussed in this study. NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatography mass spectrometry (GC-MS), and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80℃ and freeze-drying for four years. It could remain its degrading ability after 90℃ of high temperature treating for 30 min, and resume its degrading ability after subcultured 2 times after 105℃ of high temperature treating for 30 min in autoclave. It suggested that NSC-7 had efficient conservation stability and thermo-stability. DGGE analysis showed that the composition of NSC-7 remained stable after subcultured for several times.

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刘长莉,王小芬,牛俊玲,李献梅,沈海龙,崔宗均. 一组多功能细菌复合系NSC-7的培养特性及稳定性[J]. 微生物学通报, 2008, 35(5): 0725-0730

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