A bacterium strain D-1 capable of degrading DDT was isolated from the DDT contaminated soil．Based on the phenotype, physiological and biochemical characteristics, and the phylogenetic analysis of 16S rDNA sequence, the strain D-1 was identified preliminarily as Stenotrophomonas sp.. This strain could degrade DDT under condition of 30℃, pH7.0, 200 r/min, with degradation efficiency of 69.0% in 10 days.