The influences of several factors on the growth and removal for 1,2-Dichlorobenzene of Rhodobacter sphaeroidcs under phototrophic (anaerobic in light) condition were analyzed in this study. The results demonstrated that the optimum conditions for the growth of the strain and the removal rate for 1,2-Dichlorobenzene were pH 7.0, temperature at 30℃ and inoculation amount of 10% under phototrophic condition．The removal rate for 1,2-Dichlorobenzene reached 90%under the optimal conditions．Furthermore, analysis of degradation products of 1,2-dichlorobenzene by the strain tested showed that chlorobenzene, 4-hydroxybenzote were formed. The probable degradative routs taken in the anaerobic metabolism of chlorinated aromatic compounds by Rhodobacter sphaeroides were that they are metabolized anaerobically to form chlorobenzene, and then chlorobenzene was dechlorinated and passed through 4-hydroxybenzote routes for aromatic ring cleavage.