In order to change the situations such as students’ passivity and low enthusiasm in the traditional experimental teaching of Microbiology and improve students’ comprehensive qualities, exploration and attempt on experimental teaching reforms including teaching contents, teaching modes, laboratory management and evaluation system of examination were done. Teaching practices showed that the normalized experiment syllabus based on the correct orientation of experimental teaching of Microbiology, the actualization of teaching mode of basic skill training-integrated experiment-designed experiment and the management of opening laboratory were effective measures in improving experimental teaching of Microbiology.