The stress resistance of Saccharomyces cerevisiae industrial strains for molasses to highconcentration ethanol, high temperature, high osmotic pressure, furfural toxicity, phenol toxicity, acetic acid toxicity and G418 toxicity were analyzed by the spot dilution growth assays in this paper. The results showed that the stress resistances among these industrial strains were obviously different. The strains AS2.1189 and AS2.1190 are more resistant to the tested stress factors than any others .The strain 396 is the most resistant to the acetic acid toxicity and G418 toxicity, and the strain 2610 is the most resistant to the high temperature.