As a major ingredient of high medical college education, experiment practice plays an important role for medical students in integrating the medical theory with practice, training their capability of analyzing and thinking to definitively solute the problems in their study. Microbiology experiment is characterized with strong comprehensiveness and manipulation, which requests the students both aseptic operation and consciousness of bio-safety. To promote the student′s comprehensive diathesis and to improve the experiment teaching quality, several common problems of students in medical microbiology experiment practice were analyzed and discussed in this article.