Abstract:Microbial hydroxylation of androst-4-ene-3,17-dione(4AD) was investigated in this study.The hydroxylated product of 4AD,11α-hydroxyandrost-4-ene-3,17-dione (11α-OH-4AD) is valuable intermediate in the synthesis of pharmaceuticals. 30 different species and strains of fungi were screened for their biotransformation ability. It was found that Beauveria bassiana QY2A strain significantly showed the high 11α-hydroxyalate efficiency and transformate 4AD to the target product 11α-OH-4AD which had been purified and identified.The hydroxylation condition of 4AD by B. bassiana QY2A was studied.The optimum transformation conditions were obtained: the initial pH value of 6.0, temperature 28℃, 180r/min, the transformation time 60h, the concentration of solution and substrate were 2.5% and 2.5g/L respectively. Then the conversion yield of 11α-OH-4AD was 65%, increased by 51.2%.
