The isolated 24 strains-producing hydantoinase & carbamoylase were first identified by Biolog microbial identification system and 16S rDNA sequence analysis. The results suggested that the hydantoinase & carbamoyalse-producing bacteria belonged to Bacillus, Geobacillus, Brevibacillus, Aneurinibacillus, Microbacterium, Pseudomonas, Kurthia and Empedobacter, and so on. Especially, Kurthia and Empedobacter were new hydantoinase & carbamoylase-producing genera. Furthuremore, it was found that D-hydatoinase & carbamoyalse-producing bacteria belonged to Pseudomonas and Agrobacterium, while most of L-hydantoinase & carbamoyalse-producing bacterial belonged to Bacillus，Geobacillus and Microbacterium. The distribution feature of D-hydantoinase & carbamoyalse-producing bacteria and L-hydantoinase & carbamoyalse-producing bacteria showed some genera tendency. This research work will provide the biomaterial of different hydantoinase and carbamoylase and contribute to study the structure and function, molecular evolution of the two enzymes.