The natural promoter of aldehyde/alcohol dehydrogenase (AdhE) gene (adhE) from Thermoanaerobacter ethanolicus was analyzed in Escherichia coli using a gene expression reporter system. The sequence immediately upstream of adhE is recognized as a constitutive promoter in E.coli, as well as being presumably an active promoter in T.ethanolicus. The natural promoter of adhE is composed of two independent promoter regions, although activities of the two independent promoter regions are much lower than that of the natural promoter. The results indicate that the AdhE synthesis is cooperatively regulated by the two promoters in T.ethanolicus.