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微生物学通报

异常球菌007T形态和辐射抗性的研究
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Study on Morphology and Radiation Resistance of Deinococcus 007T
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    摘要:

    观察耐辐射异常球菌Deinococcus属的模式菌株Deinococcus radiodurans R1(AS1.633)和野生型异常球菌007T的生长特性和形态特征;与耐辐射模式菌株AS1.633和辐射敏感菌株 Escherichia coli DH5α 的进行平行辐射试验, 分析007T 对紫外线和γ射线辐射的抗性。结果表明,AS1.633和007T的生长特性和形态特征有所不同,AS1.633为橙红色光滑、边缘整齐的菌落,而007T为鲜红色菌落,边缘不整齐,菌落表面干燥、呈褶皱。耐辐射试验表明007T 对紫外线辐射存活最高剂量是624Jm-2,存活率为4%; γ射线16kGy照射后,007T存活率为6%,说明007T具有的极强的辐射抗性。

    Abstract:

    Studies were carried out to characterize and compare the morphology, cultivation and radiation resistance of 007T to type strain AS1.633 as well as the known radiation resistant organism Deinococcus radiodurans R1. To determine the levels of radio resistance, culture of bacteria 007T by comparing with cultures of bacteria AS1.633 and E. coli DH5α as well as the known radiation resistant and radiation sensitive organisms Deinococcus radiodurans R1 and Escherichia coli, respectively, were irradiated at the desired doses of UV and 60Co gamma radiation. Results showed some difference of the morphology and cultivation characteristics of AS1.633 and 007T. The colonies of AS1.633 were orange-red and smooth, whereas the colonies of 007T were bright red and usually dry with pleats. Strain 007T showed strongly resistant to UV and 60Co gamma radiation compared to the radiation resistant organism D. radiodurans R1 and the radiation sensitive organism E.coli DH5α, 4% survival after exposition to UV-doses as high as 624Jm-2 and 6% survival after radiated by 16kGy,respectively.

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周建琴,栾迎春,张玉琴,高凌玉,沈继红,孙承航. 异常球菌007T形态和辐射抗性的研究[J]. 微生物学通报, 2007, 34(4): 0682-0685

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