To study the dual culture relationship of fungus B-39 from Polygonum cuspidatum and suspension Polygonum cuspidatum cell and their accumulated features of resvertrol, the morphology and growth status of fungus B-39 and suspension P.cuspidatum cell and the resvertrol accumulated features were investigated. The results showed that they could normally grow in the dual culture system, and the growth of the dual cultural group was 4.908 g, but the content of resvertrol was reduced to 50μg/mL. A dynamic balanced system was established through controlling the fungal inoculums. It is presumed that fungus B-39 and suspension P.cuspidatum cell could be a balanced antagonism.