Abstract:Strain Burkholderia cepacia X4 was isolated from long time oil polluted soil,which was able to degrade oil and grease effectively.The optimal temperature and pH for oil degradation were 30℃ and 7.0 respectively.The suitable nitrogen source,C/N and Ca2+ for strain growth and oil degradation were (NH4)2SO4,4∶1 and 50mg/L respectively.Addition of co-substrate carbon source increased biomass and oil degradation rate.With proper quantities of glucose,the oil degradation rate is increased by 8%～10%.When the concentration of olive oil reach 20g/L,the maximum oil biodegradation rate can still reach 83%.Oil degradation by Burkholderia cepacia X4 followed Monod kinetics model in case of olive oil ≤2500mg/L.
