In this study, 11,150 qualified ESTs(including 10 cDNAs), about 8.1×10⁶ bp in length, were downloaded from ESTs databases of Lentinula edodes in Fungal Genomics Project(FGP) and NCBI. By searching with SSRhunter 1.3 and handwork, 469 SSRs were identified in 316 ESTs, approximately 2.83% of total ESTs. The average distance between SSRs was about 17.3kb. Trinucleotide and hexanucleotide ESR-SSRs were found to be the two most aboundent repeats, accounting for 38.00% and 20.00% of the total ESR-SSRs respectively. The most familiar motifs are(A)_n,(T)_n,(GA)_n,(AG)_n,(TGA)_n,(GAT)_n and(TCTTT)_n, which comprised about 35.94% of all EST-SSRs.