Microbiology experiment is an important professional basic course of biology majors. Microbiology experimental skills are an important way for students to know, modify and utilize microorganisms. The project-based microbiology experiment teaching mode is constructed rely on high-quality teaching resources, advanced equipment and network technology. Through the scientific design, implementation and evaluation of the project, a series of reform are carried out for experimental teaching practice and exploration, which improves the experiment teaching effect and efficiency, and makes the students have a more systematic understanding of microbiology research. This method plays a positive and effective role in cultivating the students’ comprehensive practical ability and the scientific research thinking.