[Objective] In order to identify peat bacterial diversity and community structure in Gahai Lake wetland in Gan’nan. [Methods] By Illumina miseq high-throughput DNA sequencing platforms, we studied the bacterial diversity from three samples of peat colonies. [Results] We obtained 108 096 non-redundant sequences by comparing the silva databases. The preponderant bacterium from three Gahai Lake wetland peat samples were Anawerodineaceae-uncultured, Micromonospora, Brevundimong and Nocardioides. [Conclusion] Peat bacterial diversity and community structure in Gahai Lake wetland can contribute to the development and utilization of functional bacteria.