The corn sheath blight was the main fungus pathogen of the corn. One endophytic bacteria strain which could significantly inhibit corn sheath blight and promote corn growth was isolated from Chuandan 13 corn seeding. According to morphological, biophysiological and biochemical characteristics and 16S rDNA sequence homology, the strain was identified as Bacillus subtilis, pot experiments indicated the strain could inhibit corn sheath blight from happening, the inhibit rate could reach 43.01%; promoting growth experiment revealed that it could significantly promote corn seed growth, the promoting effect was equal to that of IAA, which showed a good development prospect.