One endophytic bacteria named XJPL-YB-26 had obvious antagonistic action against Fusarium oxysporum f. sp. niveum was isolated from leaves of Pistia stratiotes L., the absorption peak of its metabolizable production is 280 nm. The part 16S rDNA was PCR using the primer designed by Primer 6.0 and sequenced. The accession of GenBank is EU251191. The 16S rDNA phylogenetic tree was constructed by comparing with published 16S rDNA sequences of the relative bacteria species. XJPL-YB-26 and AB271744 was the closest relative with 99% sequence similarity. According to the phylogenetic analysis, it was identified as Bacillus subtilis.