[Objective] In order to construct dysbacteriotic mice model of diarrhea and understand the curative effect of ultra-micro Qiweibaizhusan. [Methods] The dysbacteriotic mice models were constructed by using traditional Chinese medicine compound and combination antibiotics to cause diarrhea, and then treated with ultra-micro powder of Qiweibaizhusan. [Results] Traditional Chinese medicine dachengqitang had quick effect on diarrhea. The symptom of insufficiency of the spleen would appeared in mice with long time traditional Chinese medicine dachengqitang treatment. But it had low effect on intestinal microbes (P>0.05). The combining using of gentamycin sulfate and cefradine had the best effect on diarrhea. After treated with ultra-micro powder Qiweibaizhusan, traditional Chinese medicine dachengqitang group were cured quickly and the symptom of insufficiency of the spleen disappeared. The quantities of intestinal lactobacillus and fungus in antibiotic group were much higher than the normal group (P<0.01). [Conclusion] A dysbacteriotic mice modeling method of diarrhea was constructed. There were some kinds of probiotics contained in ultra-micro powder Qiweibaizhusan which could promote the growth of intestinal lactobacillus and fungus.