一株法夫酵母虾青素高产菌株的生产性能
Characterization and evaluation of an astaxanthin over-producing Phaffia rhodozyma
投稿时间:2010-10-25  
DOI:  
中文关键词:法夫酵母,虾青素,高产菌株,动力学,补料分批发酵
英文关键词:Phaffia rhodozyma, astaxanthin, over-producing strain, kinetic models, batch-fed fermentation
基金项目:国家自然科学基金 (No. 20702019),福建省产学研重大专项项目 (No. 2010N5009),集美大学科研创新团队基金 (No. 2010A006) 资助。
作者单位E-mail
倪辉 集美大学生物工程学院厦门 361021福建省高校食品微生物与酶工程技术研究中心厦门 361021厦门市食品与生物工程技术研究中心厦门 361021  
洪清林 集美大学生物工程学院厦门 361021  
肖安风 集美大学生物工程学院厦门 361021福建省高校食品微生物与酶工程技术研究中心厦门 361021  
李利君 集美大学生物工程学院厦门 361021  
蔡慧农 集美大学生物工程学院厦门 361021福建省高校食品微生物与酶工程技术研究中心厦门 361021厦门市食品与生物工程技术研究中心厦门 361021 chn@jmu.edu.cn 
苏文金 集美大学生物工程学院厦门 361021福建省高校食品微生物与酶工程技术研究中心厦门 361021厦门市食品与生物工程技术研究中心厦门 361021  
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中文摘要:
      为了评价虾青素高产菌株-法夫酵母JMU-MVP14的生产性能及建立虾青素高产发酵技术,通过测定糖、生物量、虾青素产量、总类胡萝卜素产量等发酵参数,用摇瓶试验对比了法夫酵母JMU-MVP14和出发菌株的差异,用7 L罐试验对比了pH值调控方式及补料培养基成分对发酵的影响,用1 m3罐试验评估了法夫酵母JMU-MVP14高密度发酵虾青素的产量水平。摇瓶发酵结果表明,法夫酵母JMU-MVP14虾青素及总类胡萝卜素的细胞产率分别达到6.01 mg/g及10.38 mg/g;7 L罐分批发酵试验结果表明,自动流加调
英文摘要:
      We evaluated an astaxanthin overproducing Phaffia rhodozyma JMU-MVP14, and developed astaxanthin high-yielding fermentation process. We analyzed several fermentation parameters, i.e., biomass, astaxanthin and total carotenoids content to compare the characteristics of P. rhodozyma JMU-MVP14 and the original strain through flask fermentation experiments. We conducted batch and fed-batch fermentation experiments in 7 L fermentor to investigate the effects of pH controlling models and feeding medium compositions on the production of astaxanthin. We further evaluated the capability and practical value of P. rhodozyma JMU-MVP14 by fed-batch cultivation in the 1 m3 fermentor. Flask fermentation experiments revealed that P. rhodozyma JMU-MVP14 produced high yield of astaxanthin and carotenoids with specific productivity of astaxanthin and specific productivity of total carotenoids of 6.01 mg/g and 10.38 mg/g. Results of batch culture experiments in the 7 L fermentor showed that controlling the pH by ammonia auto-feeding was better than discontinuously adjusting pH value at 6.0 with regard to the high productivities of biomasses and astaxanthin. This P. rhodozyma strain synthesized astaxanthin partially linked to the growth with the Ks and μmax of 0.20 h?1 and 21.73 g/L, respectively. Results of batch-fed fermentations in 7 L fermentor indicated that the complex feeding medium consisted of 50% glucose, 0.5% yeast extract and 0.3% corn steep syrup had lower astaxanthin productivity than the simple feeding medium containing only 50% glucose, which produced biomass, volumetric productivity of astaxanthin, volumetric productivity of total carotenoids, specific productivity of astaxanthin and total carotenoids at 32.81 g/L, 155.99 mg/L, 4.94 mg/g, 399.99 mg/L and 12.19 mg/g, respectively. As fed-batch cultured in 1 m3 fermentor, P. rhodozyma JMU-MVP14 yielded 85.11 g/L of biomass, 279.96 mg/L of volumetric productivity of astaxanthin, 618.01 mg/L of volumetric productivity of total carotenoids, 3.29 mg/g of specific productivity of astaxanthin and 7.26 mg/g of specific productivity of total carotenoids. Additionally, P. rhodozyma JMU-MVP14 cell contained 21.54% of protein, 41.34% of carbohydrate and 34.31% of lipid. These comprehensive results suggest that P. rhodozyma JMU-MVP14 has great practical prosperity related to its strong ability to produce astaxanthin and good value byproducts.
倪辉,洪清林,肖安风,李利君,蔡慧农,苏文金.一株法夫酵母虾青素高产菌株的生产性能[J].生物工程学报,2011,27(7):1065~1075
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