Gibberellin is one of the most important plant growth regulators, and widely used in agricultural production. However, the high cost of gibberellins production is restricting its efficient application. In recent years, biotechnological innovations have improved the synthesis of gibberellin. Gibberellin biosynthesis requires various enzymes. Current research focuses on the biosynthetic mechanisms of gibberellin and the metabolic engineering techniques to improve the production of gibberellin. This paper reviews the current research on gibberellin biosynthesis pathway, the key and enzymes environmental factors involved, and the metabolic regulation of gibberellin in Gibberella fujikuroi, summarizes the application of metabolic regulation in gibberellin biosynthesis, to provide the basis for achieving stable gibberellins production.