Multiple PCR (Multiplex polymerase chain reaction, MPCR) is a technology to simultaneously amplify multiple targets through a single reaction and to detect the amplification products by reliable detection means so as to realize the diagnosis of multiple targets. MPCR has been well studied for its high efficiency, high throughput and low cost. At present, MPCR has been widely used in scientific research, disease diagnosis and other fields. Here, we summarize the development and application of MPCR from amplification and detection, and discuss the advantages and existing problems of MPCR. We propose that separate the reaction mixture into droplets or combined MPCR with the capillary convective PCR is expect to further improve the amplification efficiency of the surface of the solid phase carrier, so as to provide reference for the development of multiple PCR with high amplification efficiency, good consistency, good stability and multiplex detection.