Bacterial ghost is intact envelope of Gram-negative bacteria, which is produced by the function of the lysis gene E from bacteriophage PhiX174. The expression of the lysis gene E is usually controlled by the thermosensitive λpL/pR-cI857 promoter. In this study, we described a mutation (T→C) at the ninth nucleotide of the OR2 in the λpR promoter of the λpL/pR-cI857 system by overlap PCR. The bacteriolytic assay showed that the mutation in the λpL/pR-cI857 system enhanced the temperature of repressing the expression of gene E up to 37 ℃. The lysis efficiency of altered λpR promoter in Escherichia coli DH5α and avian pathogenic E. coli DE17 was up to 99.9%. The expanded range of temperature will benefit for the production of bacterial ghost.