We investigated the plant regeneration and production of flavonoids in three high-yield flavonods transgenic Saussurea involucrata hairy roots C17, C27 and C46 by quantification of two phytohormones GA₃ and IAA. The results showed that GA₃ concentration at more than 1.0 mg/L could induce adventitious shoots in the hairy root lines. The highest shoot regeneration rate, about 82%, was obtained when the hairy roots C17 were cultured with 2.0 mg/L GA₃. The results on HPLC and UV spectrophotometry showed that exogenous application of both GA₃ and IAA increased the content of flavonoids in the hairy roots. The contents of flavonoids and apigenin in the hormone-treated hariy roots and regenerates were higher comparing with those in the untreated hairy roots and the regenerates. However, the content of flavonoids was not related to tissue weight, and was negatively related to the regeneration efficiency.