An appropriate evaluation mode would be an important guarantee to build what we call “A golden course” and to fully achieve the teaching objectives. We are trying to explore the proper assessment and evaluation criteria for Medical Microbiology and Parasitology Experiment course to make our teaching in a better efficiency. The research subjects included the five-year program undergraduates of medical education in Grade 2015, who were assessed by the summative assessment mode, and the five-year program undergraduates of medical education in Grade 2017, who were assessed by the comprehensive assessment mode, combining formative assessment with summative assessment. Based on the final scores and the questionnaire survey analysis, the average score of final practical exam of Grade 2017 was 87.7±9.35 and that of Grade 2015 was 82.0±9.05. The average score of Grade 2017 was higher than that of Grade 2015 with significant difference (P<0.01). Furthermore, the students’ satisfaction degree increased from 86.9% (summative assessment mode) to 98.0% (comprehensive assessment mode). In conclusion, compare to sole application of summative assessment mode in the students assessment, the invite-in of comprehensive assessment mode in our Medical Microbiology and Parasitology Experiment course has been proved to be able to improve students’ enthusiasm to active learning and to be helpful with elevating the teaching quality.