In order to strengthen the application of information technology, improve students’ information literacy and comprehensive ability, the microbiology teacher in Dalian University School of Life Science and Technology designed a new assessment method named Informatization Knowledge Contest for the first time. The microbiology course assessment formed a pattern involving “informatization technology of answering” and “receptor test”, the specific steps are as follows: first, students ask and answer question, and then the teacher “audit” assessment. The results showed that this new type of receptor appraisal method has been widely recognized by students. It is beneficial to the cultivation of students? comprehensive ability and improvement of students’ information literacy.