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Advances in the functions of African swine fever virus-encoded proteins
African swine fever (ASF) is an acute, hemorrhagic and severe infectious disease caused by African swine fever virus (ASFV) in domestic or wild pigs, which is characterized by short course, high fever and hemorrhagic lesions as well as 100% mortality rate of acute infection. So far, there is no effective vaccine available for ASF. ASFV is a large, double stranded DNA virus and the only member of the Asfarviridae family, Asfivirus genus, which rep...
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Prokaryotic expression of truncated S2 subunit of porcine epidemic diarrhea virus and identification...
[Background] Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) has caused significant economic losses in pig industry. PEDV S protein can induce the host to produce neutralizing antibodies. [Objective] To prokaryotically express the truncated S2 (aa: 961?1 382) of PEDV CV777 vaccine strain and prepare polyclonal antibodies with which to identify regions containing linear B cell epitopes on the truncated S2. [Methods...
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Analysis of interaction mode between Escherichia coli and Staphylococcus aureus by systems mapping
[Background] The interaction between species is an important driving force for the evolution of species. But it is challenging to determine the single nucleotide polymorphism (SNPs) that play an important role in the process of interaction between species and establish an accurate genotype-phenotype map. [Objective] To obtain the significant SNPs that play an important role in the interaction between Escherichia coli and Staphylococcus aureus and...
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Construction and characterization of Burkholderia pseudomallei bopA gene knockout mutant
[Background] Burkholderia pseudomallei is an intracellular parasitic bacterium that can cause human disease and even death. The type III secretory system plays an important role in the bacterial invasion of epithelial cells, escaping host immunity and the secretion of virulence factors. The bopA gene is an important effector protein encoded by the TTSS-3 gene, and it plays an important role in the immune escape of the Burkholderia pseudomallei. [...
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Effect of N-glycosylation on the stability of a novel recombinant thermostable β-mannanase (ReTMan26...
Abstract: [Background] Based on the sequence analysis of a novel recombinant highly thermostable ?-mannanase (ReTMan26) from a thermophilic Bacillus subtilis (TBS2), there are 3 N-glycosylation sites (N8, N26 and N255) in the encoding gene of ReTMan26, and ReTMan26 could be N-glycosylated when expressed by Pichia pastoris. [Objective] To determine the effects of N-glycosylation on the stability of 12 微生物学通报 Microbiol. China Tel: 010-64807511; E...
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Synergism between ethanol and acidity drives microbial community succession during roasted sesame-fl...
Abstract: [Background] The assembly dynamics of microbial community and its environmental driving forces during liquor solid-state fermentation (SSF) is important for revealing the characteristics of brewing microbes. [Objective] The purpose of this study was to reveal microbial community succession and its environmental driving forces during roasted sesame-flavor liquor SSF. [Methods] The dominant genera and community structure transition were r...
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