Flowing Rock
Expression and characterization of succinic semialdehyde dehydrogenase from Cyanothece sp. ATCC51142...
[Background] The cyanobacterial tricarboxylic acid cycle, which produces succinic acid, is different from that of other species. The existences of α-ketoglutarate decarboxylase and succinic semialdehyde dehydrogenase in cyanobacterium make its tricarboxylic acid cycle complete. Succinic semialdehyde dehydrogenases that catalyze the conversion of succinic semialdehyde to succinic acid, exist in cyanobacteria extensively. [Objective] Clone, express...
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Flowing Rock
Effect of metal ions on the activity and stability of recombinant cold-adapted α-amylase LamA from L...
[Background] Luteimonas abyssi XH031 is one kind of marine bacteria with strong starch degradation ability. Previous studies showed that LamA, a cold-adapted α-amylase identified from strain XH031, kept high activity under low temperature. LamA will have great application prospects if the high temperature tolerance is improved. [Objective] To determine the calcium ion-dependent thermo-stability enhancement mechanism of LamA, site-directed mutagen...
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Flowing Rock
Analysis of genome sequence and natamycin biosynthetic gene cluster on high producing strain Strepto...
[Background] Natamycin is a natural, broad-spectrum and efficient polyene macrolide antifungal antibiotic. Streptomyces gilvosporeus is an important natamycin-producing bacterium. However, the genome sequence analysis of S. gilvosporeus has not been reported until now, limiting studies on the biosynthesis and regulation of natamycin and other secondary metabolites in S. gilvosporeus. [Objective] The genomic sequence information of the S. gilvospo...
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Flowing Rock
Effects of cleavage sites of leader peptides on the activities of class Ⅱ lantibiotic peptidases
[Background] Class Ⅱ lantibiotics are ribosomally synthesized and post-translationally modified peptides, mainly produced by Gram-positive bacteria. In the last step of its biosynthesis, the N-terminal peptidase domain of transporter protein LanT cleaves the leader peptide to produce active lantibiotics. However, the removal mechanism of leader peptide in this class lantibiotics is still not clear. [Objective] To investigate the effects of cleava...
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Flowing Rock
Prokaryotic expression of truncated S2 subunit of porcine epidemic diarrhea virus and identification...
[Background] Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) has caused significant economic losses in pig industry. PEDV S protein can induce the host to produce neutralizing antibodies. [Objective] To prokaryotically express the truncated S2 (aa: 961?1 382) of PEDV CV777 vaccine strain and prepare polyclonal antibodies with which to identify regions containing linear B cell epitopes on the truncated S2. [Methods...
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Flowing Rock
Advances in the functions of African swine fever virus-encoded proteins
African swine fever (ASF) is an acute, hemorrhagic and severe infectious disease caused by African swine fever virus (ASFV) in domestic or wild pigs, which is characterized by short course, high fever and hemorrhagic lesions as well as 100% mortality rate of acute infection. So far, there is no effective vaccine available for ASF. ASFV is a large, double stranded DNA virus and the only member of the Asfarviridae family, Asfivirus genus, which rep...
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