Screening, identification and fermentation optimization of a newly isolated raw starch digesting amy...
[Background] Raw starch digesting amylase can directly degrade raw starch granules below the gelatinization temperature. Hence, it has been wildly used in the production of bioethanol, Chinese spirit, yellow rice wine and vinegar. [Objective] This study aims at isolation and identification new raw starch digesting amylase-producing strain, to provide enzyme resource that can be used in the process of fermentation with uncooked materials. [Methods...For details
Influence of Tyr78-loop on phenylalanine ammonia-lyase from Anabaena variabilis
[Background] Enzymatic property of the MIO-dependent (methylidene-imidazol-5-one) enzyme is significantly affected by the flexibility of the special loop that includes the catalysis Tyr (Tyr78-loop). [Objective] The effect of Tyr78-loop on activity of phenylalanine ammonia-lyase (PAL) from Anabaena variabilis was investigated to improve PAL catalytic activity. [Methods] Tyr78-loop was genetically modified. Positive mutants with enhanced activity ...For details