Bioinformatics courses aim to introduce computational technology to the scientific community focusing on animal, plant and microbiology, involving analytic program development and its application. With the rapid expansion of life science, the scope of bioinformatics teaching is continuously suffering a variety of improvements. The teaching mode and development facilitated by customized microbial informatics programs were presented in this report. Graduate students’ innovation and abilities were promoted by interactive platform-driven strategy. The content arrangement, teaching style, presentation methods and scoring criterion have been optimized to satisfy graduate academic prerequisites. These beneficial experiences could assist in improving the quality of talent cultivation.