Microorganism and Life is the optional course of general education elective course in the department of bioengineering of Hubei University of Technology, which has been opened for 5 years. Here we summarized the practice of reformed teaching methods, emotion training strategy and multiple course evaluation system. The modular teaching and integration of classroom teaching and social practice were used to enhance the students’ learning efficiency and facilitate students’ learning ability. The written-course-complete report and oral presentation were adopted to evaluate the study effect instead of the conventional examination mechanism. The students’ learning enthusiasm was greatly improved via improvement of the teaching modes. Five-year’s teaching reforming practice has played an important role in improving the quality of students. Meanwhile, the students were encouraged to be more interested in scientific research. It was proved that the course Microorganism and Life made a great achievement. In this paper, we discussed the effective ways of knowledge transmitting, affective education integrated theoretical teaching methods, and our productive practice and experience.