A small private online course (SPOC) is a kind of blended teaching mode that combines the resources of a high-quality massive open online course (MOOC) with face-to-face classroom teaching. It is the reconstruction and innovation of teaching mode. Based on this concept, we have put into practice of the “SPOC-classroom” teaching mode of Microbiology course, starting from the design of teaching plan, the implementation of “SPOC-classroom” teaching, to the multi-dimensional score evaluation ways. By empolying advanced teaching methods and high-quality teaching contents, SPOC has significantly aroused students’ interest in learning and improved the teaching effect of Microbiology course.