Microbial Engineering Technology and Equipment is a core course in bioengineering majors, which highly emphasizes the development of practical knowledge and applied skills. The theory of outcome-based education (OBE) was adopted necessarily in this college course teaching reform emphasized in three key points: 1) to clarify the ability of the students in the course and then formulate the expected learning outcomes; 2) to optimize the entirely teaching process, including the integration of instructional contents, and finally a multiple complex teaching-learning platforms of “Class theory teaching – Learning in the network – On site teaching – Computer simulation” would be constructed successfully; 3) to diversify student-orientated pragmatic and real-time evaluation methodology to promote continued improvement of instruction and educational alignment. Through these reforming strategies, we could leverage the students self-paced learning skills, and thereby promote a higher level of excellence in college biology curriculums.