The cyclodextrin glycosyltransferases (CGTases) are enzymes that used to synthesize cyclodextrin, and this method of production of cyclodextrin is mostly used at present. we described several engineering strains which were used to produce CGTase including Escherichia coli, Bacillus subtilis and Pichia pastoris, and the expression system of E. coli is one of the most widely used. In addition, we also summarized the effective strategies for high efficient expression of CGTase including appropriate expression vector, promoter and signal peptide, codon optimization, co-expression with molecular chaperone. The purpose of this review is to provide a reference for relevant research of CGTase.