光强及氮浓度对丝状绿藻双星藻生长及生化组成的影响
Effects of light intensity and nitrogen concentration on the growth and biochemical composition of filamentous green alga Zygnema sp.
  
DOI:  10.13344/j.microbiol.china.190171
中文关键词:丝状绿藻,双星藻,生物量,生化组成,脂肪酸组成
英文关键词:Filamentous green alga, Zygnema sp., Biomass, Biochemical composition, Fatty acid composition
基金项目:国家高技术研究发展计划(2013AA065805);广东省供水水库蓝藻水华应急处置装备与技术项目(2015B020235007);石油化工科学研究院科研项目(ST18005-2)
作者单位E-mail
戴晨明 暨南大学生态学系水生生物研究中心 广东 广州 510632  
高保燕 暨南大学生态学系水生生物研究中心 广东 广州 510632  
苏敏 暨南大学生态学系水生生物研究中心 广东 广州 510632  
黄罗冬 暨南大学生态学系水生生物研究中心 广东 广州 510632  
王飞飞 暨南大学生态学系水生生物研究中心 广东 广州 510632  
赵伟 暨南大学生态学系水生生物研究中心 广东 广州 510632  
张成武 暨南大学生态学系水生生物研究中心 广东 广州 510632 tzhangcw@jnu.edu.cn 
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中文摘要:
      【背景】环境因子和营养因子对微藻的生长和生化组成都有显著的影响,其中光强和氮浓度是最重要的两个条件。【目的】研究不同光强和初始氮浓度对丝状绿藻-双星藻(Zygnema sp.)生长及生化组成的影响。【方法】采用改良的BBM培养基,设置了两组光强[100 μmol/(m2·s)和300 μmol/(m2·s)]和6种初始氮浓度(3、6、9、12、15和18 mmol/L)在柱状光生物反应器中对双星藻进行培养。【结果】在高光强条件下[300 μmol/(m2·s)],12 mmol/L初始氮浓度最有利于双星藻生物质的积累,其最高生物量可以达到6.60 g/L,而初始低氮浓度(3 mmol/L)则促进了油脂和脂肪酸的积累,油脂最高含量占干重的32.13%,且脂肪酸组成主要包括棕榈酸(C16:0)、油酸(C18:1)、亚油酸(C18:2)和亚麻酸(C18:3),其中油酸含量最高达到总脂肪酸含量的55.01%;在低光强条件下[100 μmol/(m2·s)],初始氮浓度为18 mmol/L时,总蛋白质和总碳水化合物的含量达到最高,分别占干重的16.35%和37.70%,而总脂含量仅占干重10.16%。【结论】光强和初始氮浓度对双星藻生长具有较大影响,通过调节光强和初始氮浓度可有效提高双星藻目标代谢产物的积累。
英文摘要:
      [Background] Environmental factors and nutrient factors have significant effects on the growth and biochemical composition of microalgae, and light intensity and nitrogen concentration are two of important environmental factors. [Objective] The effects of different light intensities and initial nitrogen concentrations on the growth and biochemical composition of filamentous green alga, Zygnema sp. was investigated. [Methods] The alga was grown in bubble column glass photobioreactor with modified BBM medium under two light intensities (100 μmol/(m2·s) and 300 μmol/(m2·s)) and six initial nitrogen concentration gradients (3, 6, 9, 12, 15 and 18 mmol/L). [Results] The highest biomass was observed at high light intensity (300 μmol/(m2·s)) and 12 mmol/L initial nitrogen concentration, which was 6.60 g/L. The initial low nitrogen concentration (3 mmol/L) was conducive to the accumulation of total lipid and fatty acids of Zygnema sp., and the highest total lipid content reached 32.13% of dry weight. The main components of fatty acid were palmic acid, oleic acid, linoleic acid and α-linolenic acid, and oleic acid content accounted for 55.01% of total fatty acid. Under low light intensity (100 μmol/(m2·s)), the highest protein and carbohydrate content was obtained under 18 mmol/L initial nitrogen concentration, which was 16.35% of dry weight and 37.70% of dry weight, respectively. Meanwhile, the total lipid content accounted for only 10.16% of dry weight. [Conclusion] The light intensity and initial nitrogen concentration had a great influence on the growth and biochemical composition of Zygnema sp., the target accumulation of metabolites of Zygnema sp. could be effectively increased by adjust the light intensity and initial nitrogen concentration.
戴晨明,高保燕,苏敏,黄罗冬,王飞飞,赵伟,张成武.光强及氮浓度对丝状绿藻双星藻生长及生化组成的影响[J].微生物学通报,2020,47(1):172~181
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