一株新型苯胺蓝降解菌MP-13的代谢特征
Degradation characteristics of a novel aniline blue-discoloring bacterial strain MP-13
  
DOI:  10.13344/j.microbiol.china.190414
中文关键词:三苯甲烷类染料,苯胺蓝,土白蚁特拉布尔氏菌,降解途径
英文关键词:Triphenylmethane dye, Aniline blue, Trabulsiella odontotermitis, Degradation pathways
基金项目:国家自然科学基金(31772529);江苏省研究生科研与实践创新计划项目(KYLY16_0910);江苏省高校优势学科建设工程资助项目(4013000019)
作者单位E-mail
李锋 江苏大学环境与安全工程学院生物质能源研究所 江苏 镇江 212013  
梁念 江苏大学环境与安全工程学院生物质能源研究所 江苏 镇江 212013  
孙建中 江苏大学环境与安全工程学院生物质能源研究所 江苏 镇江 212013 jzsun1002@ujs.edu.cn 
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中文摘要:
      【背景】三苯甲烷类染料的广泛使用对我国生态环境构成了极大危害,亟待开发一种经济、高效和环境友好型的染料废水处理技术。目前,利用微生物处理染料废水被认为是一种环境友好型的方法。【目的】通过解析菌株MP-13对苯胺蓝的降解特性,为该菌株在染料废水治理中的应用提供核心理论与技术依据。【方法】从食木白蚁肠道中筛选一株苯胺蓝脱色菌,对其进行16S rRNA基因序列分析鉴定,确定其基本生物学特征,然后通过FTIR、GC/MS等分析手段解析该菌对苯胺蓝的降解特征。【结果】菌株MP-13经鉴定为土白蚁特拉布尔希氏菌(Trabulsiella odontotermitis),该菌对苯胺蓝降解的最适温度、pH和转速分别为35 °C、8.0和180 r/min,苯胺蓝浓度为200 mg/L时最大脱色率可达97.3%,且对苯胺蓝的耐受浓度可达1 500 mg/L。此外,FTIR和GC/MS的结果表明苯胺蓝被降解为小分子芳香族化合物。【结论】Trabulsiella odontotermitis MP-13对苯胺蓝有较强降解能力和较高耐受性,可作为染料废水生物修复的潜在菌株资源。
英文摘要:
      [Background] The wide use of aniline blue, a common refractory organic pollutants, harms and threatens the ecological environments. Hence, it is important to develop an eco-friendly and cost-effective technology to treat the dye contaminated wastewater. Biological method is environmentally friendly and applied to deal with dye contaminated wastewater. [Objective] Our study was to provide a core theory and supporting technology to isolate and characterize a novel bacterial strain, Trabulsiella odontotermitis MP13, for its potential decolorization of dye wastewater. [Methods] Some aniline blue-decoloring bacteria were first isolated from the gut of a termite species, Microtermes pakistanicus, and then identified by 16S rRNA gene sequence analysis for their phylogeny property and other relevant biological properties. Further, with a variety of evaluation methods, such as FTIR and GC/MS, the degradation characteristics of aniline blue by strain MP13 were analyzed. [Results] A novel bacterial strain MP-13 was identified as Trabulsiella odontotermitis that could tolerate and effectively degrade aniline blue up to a high concentration of 1 500 mg/L. The optimal temperature, pH and rotation speed for dye decolorization were 30 °C, 8.0, and 180 r/min, respectively. The decolorization efficiency was up to 97.3%, when aniline blue concentration was at 200 mg/L. Further, the FTIR and GC/MS analysis showed that aniline blue was biotransformed into low molecular weight aromatic compounds. [Conclusion] Trabulsiella odontotermitis MP-13 exhibited a remarkable decolorization ability for aniline blue, suggesting its potential for industrial dye waste water treatment.
李锋,梁念,孙建中.一株新型苯胺蓝降解菌MP-13的代谢特征[J].微生物学通报,2020,47(1):43~53
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