铁锰氧化物提高巴斯德梭菌电子输出率
Iron and manganese oxides enhance electron output efficiency of Clostridium pasteurianu
  
DOI:  10.13344/j.microbiol.china.190191
中文关键词:巴斯德梭菌,电子输出率,电化学活性,铁锰氧化物,异化金属还原,发酵
英文关键词:Clostridium pasteurianum, Electron output efficiency, Electrochemical activity, Iron and manganese oxides, Dissimilatory metal reduction, Fermentation
基金项目:国家自然科学基金(91751112,41573071);中国科学院科学号高端用户项目(KEXUE2018G01);山东省自然科学杰出青年基金(JQ201608);“泰山学者”青年专家计划(TSQN20161054)
作者单位E-mail
刘进超 1 中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室 山东 烟台 264003
2 中国科学院大学 北京 100049 
 
刘芳华 1 中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室 山东 烟台 264003 fhliu@yic.ac.cn 
张月超 1 中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室 山东 烟台 264003
2 中国科学院大学 北京 100049 
 
孟德龙 3 中国农业大学烟台研究院 山东 烟台 264670  
王学明 3 中国农业大学烟台研究院 山东 烟台 264670  
肖雷雷 1 中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室 山东 烟台 264003  
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中文摘要:
      【背景】发酵型异化金属还原菌通过发酵获取能量,同时也具有一定的异化还原变价金属氧化物的能力,关于变价金属氧化物对发酵型异化金属还原菌电子输出率的影响还知之甚少。【目的】探究铁锰氧化物(Fe2O3/MnO2)对发酵型异化金属还原菌Clostridium pasteurianum电子输出率的影响。【方法】将不同浓度Fe2O3/MnO2添加到以葡萄糖为底物并接种5% C. pasteurianum的发酵体系中,利用电化学工作站检测C. pasteurianum电化学特性;以菲啰嗪(Ferrozine)显色法和甲醛肟法分别测定发酵体系中Fe(Ⅱ)、Mn(Ⅱ)含量;气相色谱、高效液相色谱检测发酵底物葡萄糖及代谢产物(乙酸、丁酸、CO2和H2)随发酵时间的变化情况;最后计算发酵过程的电子输出率。【结果】研究表明,接种C. pasteurianum的微生物燃料电池可以检测到电流的产生,最大电流密度为0.93 mA/m2;随着发酵时间的推移,反应体系中Fe(Ⅱ)和Mn(Ⅱ)的浓度逐渐增高;Fe2O3/MnO2的添加使发酵体系中葡萄糖消耗量提高了9.4%/7.7%,同时,乙酸产量提高了37.5%/25.0%,丁酸产量提高了22.7%/6.8%,氢气产量提高了21.6%/9.8%,而总的电子输出率则提高了24.27%/10.82%;添加铁锰氧化物的实验组pH值与对照组相比无显著差异。【结论】铁锰氧化物的添加可以提高C. pasteurianum的电子输出率,其原因可能是增加了葡萄糖消耗和缓冲pH值。研究结果为揭示变价金属氧化物影响发酵型异化金属还原菌电子输出的规律提供了证据,并进一步拓展了对变价金属氧化物与发酵型异化金属还原菌之间相互作用机制的认识。
英文摘要:
      [Background] Fermentative dissimilatory metal-reducing bacteria, which are capable of reducing metallic oxides, get energy from fermentation. Little is known about how metallic oxides affect electron output efficiency of fermentative dissimilatory metal-reducing microorganisms. [Objective] This study was conducted to explore the influence of iron and manganese oxides (Fe2O3/MnO2) on electron output efficiency. [Methods] Different concentrations of Fe2O3/MnO2 were added into fermented system containing glucose and inoculated 5% C. pasteurianum. Electrochemical activity of C. pasteurianum was detected. The concentrations of Fe(Ⅱ) and Mn(Ⅱ) were measured by ferrozine spectrophotometry and formaldoxime method. Fermentation substrate and metabolites of C. pasteurianum were detected by gas chromatography and high performance liquid chromatography. Lastly, we calculated the electron output efficiency. [Results] The current density peaked with the value of about 0.93 mA/m2. The concentrations of Fe(Ⅱ) and Mn(Ⅱ) gradually accumulated. The consumption of glucose was increased by 9.4%/7.7%, Meanwhile, acetate production was increased by 37.5%/25.0%, and butyrate production was increased by 22.7%/6.8%. Additionally, hydrogen production was increased by 21.6%/9.8%, and the total electron output efficiency was increased by 24.27%/10.82%, respectively. The pH values between experimental group and control are no significant difference. [Conclusion] This study shows that iron and manganese oxides can improve the electron output efficiency of C. pasteurianum by increasing glucose consumption and buffering pH value. The results provide evidence for revealing the effects of multivalent metal oxides on the electron output of fermentative dissimilatory metal-reducing bacteria, and further expand our understanding of the interaction mechanism between multivalent metal oxides and fermentative dissimilatory metal-reducing bacteria.
刘进超,刘芳华,张月超,孟德龙,王学明,肖雷雷.铁锰氧化物提高巴斯德梭菌电子输出率[J].微生物学通报,2020,47(1):24~34
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