Recently, it is the one of the hot topic for antimicrobial peptides (AMPs) to be produced using recombinant expression system with efficiency and stable protocols. It is particularly crucial for us to know different expression systems we use and their production. For this purpose, we narrate methods to produce AMPs, species, elements and properties of different expression systems, mainly there is an analysis for some difficult factors to construct engineered bacteria. And, we also reviewed the progress of producing antibacterial peptides using engineered bacteria in recent years.