Modern informational social calls for the new pattern of microbiological experiment teaching reform. The microbiology experiment teaching of cultivating students’ innovative ability is fully implemented. Based on the characteristics of students’ personality, academic performance, and hobbies, etc., refined and individualized groups were built before the class. Moreover, The main network of modern information teaching technology such as Classroom, Micro Tas, Rain Classroom, UMU and Optimization of Master Class were introduced in the Microbiology Experiment teaching and carried out for one-year pilot reform. According to the individualized characteristics of the students, five mainstream teaching platforms were carried out in the teaching of five different “Microbiology Experiment” groups. The results of the reform showed that the blended interactive teaching with refinement, individuation and network, which could promote the transformation from “qualitative analysis” to “precision quantitative analysis”. Students were deeply aware that individualized practical learning with the elaboration of teaching methods and tools had more advantages. The learning interest was stimulated and learning effect were promoted as well as comprehensive innovation ability of microbiology experiment was improved in students.