Enzyme Engineering is one of major courses of bioengineering and biotechnology major, and is the bond among these courses of Microbiology, Biochemistry and Molecular Biology, Cell Engineering and Genetic Engineering, Fermentation Engineering and Chemical Engineering, it has very strong theory and practicality. To improve the quality of undergraduate teaching, strengthen the autonomous learning ability of students, and adapt to the society that needs comprehensive talents, combined with the practical teaching inside and outside the classroom and the development of information technology under the background of “Internet+”, this paper performed practice and thinking of diversified teaching reform in Enzyme Engineering.