PAD (Presentation-Assimilation-Discussion) class is a new kind of teaching model which is not only different from the traditional teaching, but also different from the flipped classroom and the seminar style teaching. It has been included in the training of new teachers in Shanghai Education Commission and Excellent teaching method series of online training center of Ministry of Education. The author introduced the PAD class into the Biology teaching firstly in 2015. After the application of 4 semesters in two courses, questionnaire surveys were carried out in an anonymous manner. 106 valid questionnaires were gotten. It shows the application effect of the PAD model on Biology teaching and will provide a new idea and a new model for peers to improve the quality of classroom teaching.