科微学术

微生物学通报

黄曲霉和米曲霉的多相鉴定方法
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家重点研发计划(2016YFD0400500)


The polyphasic taxonomy for distinguishing Aspergillus flavus and A. oryzae
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    【背景】黄曲霉(Aspergillus flavus)和米曲霉(Aspergillus oryzae)形态特征相近,基因组高度相似,较难区分。【目的】旨在总结一套准确鉴别二者的分类方法。【方法】利用22株标准菌株对传统形态学、产毒培养基、酶联免疫毒素检测、系统发育分析、产毒基因检测等5种鉴别方法分别进行验证。【结果】各鉴定方法的结果存在异同,单一的鉴定方法容易出现假阴性或假阳性结果。【结论】利用单一方法区分黄曲霉和米曲霉具有潜在风险,多相鉴定方法可以准确鉴别二者。

    Abstract:

    [Background] The morphology and genome between Aspergillus flavus and A. oryzae were highly similar, which made it very difficult to distinguish with each other. [Objective] The aim of this study was to establish an accurate method for distinguishing A. flavus with A. oryzae. [Methods] Twenty-two standard strains were used to study the five traditional identification methods of A. flavus and A. oryzae, including the traditional morphological identification method, enzyme linked immunosorbent assay (ELISA), the toxin-secreting culture medium method, the phylogenetic tree method and the detection method for toxin genes. [Results] There are differences or complements among the results of different methods. [Conclusion] A single method used to distinguish the two species would have a potential risk, while a polyphasic approach could resolve this problem.

    参考文献
    相似文献
    引证文献
引用本文

白飞荣,姚粟,凌空,程池. 黄曲霉和米曲霉的多相鉴定方法[J]. 微生物学通报, 2018, 45(1): 215-226

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2018-01-05
  • 出版日期: