Brucellosis is a zoonotic and contagious infectious disease caused by infection with Brucella species, threat to livestock and human healthy seriously. Prevent and eliminate to brucellosis is significant to public health security and development of breeding industry. However, the pathogenic mechanism and relative virulence factors are not clear due to the facultative intracellular pathogen and complex construction for Brucella. In addition, brucellosis vaccine are all smooth strains currently in China, leading interfere with natural infection, which cause the seriously difficult for purification. Most of research had not preferable result due to these problems. Proteomic is a new method which is developing gradually to study constitutes and disciplinarians of proteins, the researches have increased to reveal the pathogenesis and immunologic mechanism of Brucella, and supplied a new way to solve above problems. Based on the author’s own research in this field, this paper makes a summary on research progress of proteomic in the brucellosis research for seek the specific proteins and differential diagnosis methods.