[Objective] In recent years, the epidemic of infectious bronchitis virus in China has become a popular trend in domestic flocks, especially the emergence of mutant strains, which aggravate the harm to the flocks. Major histocompatibility complex (MHC I) class I is able to bind antigens containing certain motif and further recognize and eradicate cells affected by virus. Identification of the binding motif of the complex of chicken MHC I protein BF2*15. [Methods] The complex of chicken MHC I protein BF2*15 and Nucleoprotein of infectious bronchitis virus (IBV) octapeptide were constructed by computational method, including homology modeling, molecular dynamics simulation and molecular docking. [Results] According to the interaction relationship of BF2*15 and IBV Nucleoprotein peptide, we conclude that “x-Arg-x-x-x-Arg” may represent a potential binding motif of BF2*15. [Conclusion] Interactions of the major histocompatibility complex class I BF2*15 and cytotoxic T lymphocyte epitopes from nucleoprotein of avian infectious bronchitis virus was illuminated in this study, and the results may shed light on the understanding of immune mechanism of IBV and research towards universal IBV vaccine.